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The GPX assay buffer, NADPH, GSH, glutathione reductase together with 12 g of the cellular protein samples, were added into each well of a 96-well plates and mixed well
These are not only damaging, but they can also single-handedly deplete glutathione, creating an imbalance between Phase 1 and Phase 2 (see below) activity.7 Toxic reactions can occur due to the buildup of reactive intermediate forms resulting from phase 1 detoxification, so further work needs to be done to process and eliminate toxins
(2024) Am J Clin Nutr, 120: 80.(274)Cai Y, et al
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