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While these compounds work through different mechanisms, combining multiple substances always warrants caution
The membranes were blocked with 5% skim milk in TBST, followed by overnight incubation with primary antibodies at 4 C, and subsequent incubation with HRP-conjugated secondary antibodies for one hour at room temperature
any changes that occurred were most likely due to the RT program
10.1152/ajplung.00379.2016 95 TzotzosS
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