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Details on the isolation of mouse caSMCs and neonatal ventricular CMs, as well as cell culture methods for human caSMCs, caECs, hESC-CMs, and the HL-1 cell line, including drug treatment regimens, are provided in the Supplemental Methods

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MiR-24-3p was reported to achieve this by blocking the expression of annexin A6 ( ANXA6 ) [82], and miR-128-3p was shown to inhibit their differentiation by downregulating farnesyl diphosphate synthase ( FDPS ) expression, although the specific target genes that promote proliferation remain unidentified [83]