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The human samples used in this study were acquired from a by-product of routine care or industry
The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)
J Pediatr Urol
This process ultimately culminates in menopause, the permanent cessation of ovarian endocrine and reproductive function, typically around the age of 51 (Peacock et al., 2025)
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