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Mice were given food and water ad libitum and housed in the UMSOM vivarium on a 12-h light/dark cycle
This concentration produces clean, round numbers for the starting and mid-range doses, which reduces measurement errors
Visible weight loss usually becomes apparent after 4-8 weeks
Then rotate within it exactly the way you would anywhere else: move at least a finger-width from yesterday, work through the available spots in a fixed order rather than at random, and let several days pass before any single spot repeats
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